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The feasibility of fingerstick blood collection for point-of-care HIV-1 viral load monitoring in rural Zambia, Global Health Innovation


Viral load monitoring for HIV treatment is recommended but not feasible in many settings. A point-of-care test using capillary blood would increase access butmay require up to 200 μLof blood to achieve a lower limit of detection of 1000 copies/mL.This cross-sectional study evaluated the feasibility of collecting 200 μL of capillary blood as well as blood collection preferences among adults in rural Zambia. Adults seeking HIV counseling and testing at Macha Hospital were recruited in 2015. Capillary blood was collected in four 50 μL tubes. Blood collection was categorized as complete (200 μL collected), partial (all tubes filled but <200 μL obtained due to collection techniques), or incomplete (1-4 tubes attempted;<200μLobtained due to insufficient blood flow).One fingerstickwas required for 90%of the 201 participants.Amedian blood volume of 196 μL was collected. Complete, partial and incomplete collection was achieved in 34%, 59% and 6% of participants. The majority of participants (95%) preferred fingerstick over venous blood collection. A point-of-care viral load test requiring up to 200 μL of blood is feasible in a rural setting but would require training and supervision to ensure that sufficient blood was collected.




Kara Palamountain, Mark J. Fisher

Date Published



Palamountain, Kara, and Mark J. Fisher. 2018. The feasibility of fingerstick blood collection for point-of-care HIV-1 viral load monitoring in rural Zambia. Global Health Innovation. 1(2): Article 3.


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